Effect of NS398 on anti-proliferation and inducing apoptosis of human osteosarcoma cell MG-63 line / 中国癌症杂志
China Oncology
; (12)2001.
Article
de Zh
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| ID: wpr-542861
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WPRO
ABSTRACT
Background and Purpose:Cyclooxygenase-2(COX-2) plays an important part in tumor genesis,growth,and angiogenesis.Many inhibitors of COX-2 could inhibit proliferation and induce apoptosis of cancer cells. This study investigated the impact of NS398 on anti-proliferation and the induction of apoptosis in human osteosarcoma cell line MG-63.Methods:Cell proliferation is measured by MTT method.Characteristic changes of apoptosis in morphology are observed by fluorescence microscopy、transmission electron microscopy(TEM) and quantitatively by TDT-mediated dUTP-biotin nick end-labeling(TUNEL) assay.The apoptotic rates are calculated by flow cytometry(FCM).Results:The growth inhibition rates of MG-63 cells treated with 1,10,50,100 and 200 ?mol/L NS398 are 14.7%,23.5%,33.6%,52.5% and 81.4%,respectively(P
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Indice:
WPRIM
langue:
Zh
Texte intégral:
China Oncology
Année:
2001
Type:
Article