Effect of perforin-mediated cytotoxicity on systemic defense mechanisms against primary influenza virus infection / 中国免疫学杂志
Chinese Journal of Immunology
;
(12)1985.
Article
Dans Chinois
| WPRIM
| ID: wpr-543071
ABSTRACT
Objective:
To investigate the effect of perforin-mediated cytotoxicity in primary influenza virus infection.Methods:
Perforin-deficient and wild-type C57BL/6 mice were infected intranasally with influenza virus A/PR/8/34. Pulmonary viral growth was determined at various days after infection by pfu experiment. Perforin-mediated apoptotic degeneration was observed by Immunohistochemical staining. LDH-release method was used for detection of specific CTL and NK cell activity from spleen cells.Results:
Mice deficient in the perforin gene showed an increased virus growth and prolonged virus shedding. The appearance of apoptotic degeneration in virally infected lung cells was delayed in perforin-deficient mice. The cytolytic activities of natural killer cells and virus-specific cytotoxic T lymphocytes were significantly lower than that of wild-type mice.Conclusion:
Perforin plays a critical role in the host defense system against primary influenza virus infection.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Chinese Journal of Immunology
Année:
1985
Type:
Article
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