The effect of TFu on the apoptosis and proliferation of FRH-0201 cholangiocarcinoma cell line / 中国现代普通外科进展

ABSTRACT

Objective:To investigate the effect of TFu on FRH-0201 cell and to test new drugs for the chemotherapy of cholangiocarcinoma.Methods:Morphological changes were observed by light microscopy and fluorescence microscopy.The effect of TFu on FRH-0201 cell was examined by clone formation.Cell cycle and apoptosis were examined by flow-cytometric analysis.DNA ladder was used to detect apoptosis.To compare the drug susceptibility of TFu with 5-Fu and several other common drugs by MTT colorimetric assay.Results:TFu significantly inhibited the proliferation and clone formation of FRH-0201 cell line within concentration of 0.016~4mmol/L.The inhibition ratio was dependent on ascending concentration and time,and that is higher than 5-Fu with the same concentration.The morphological character of apoptosis was observed.Flow cytometric analysis indicated that TFu induced a G_1 phase arrest and apoptosis more than 5-Fu.ADM,DDP and TFu were the sensitive drugs to FRH-0201 cel line,and the combination of them can reach the highest inhibition ratio.Conclusion:These results suggests that TFu can more significantly inhibited the proliferation of FRH-0201 cell line than 5-Fu.The mechanism may be related to the cell cycle arrest in association with apoptosis,which may provide a new approach for treating cholangiocarcinoma.