Experimental study of DFMO on the growth and apoptosis in bladder carcinoma cells / 肿瘤研究与临床

ABSTRACT

Objective An experimental study was undertaken to observe the effects of DFMO on the growth and apoptosis in bladder carcinoma EJ cells. Methods The methods of cell culture, living camera technique, eosin dying cell counting and DNA end labeling in situ were used to observe the effects of DFMO on the growth and apoptosis in bladder carcinoma EJ cells. Results Living camera technique showed that cell growth was significantly inhibited after five days of 4 mmol/L, 6 mmol/L, 8 mmol/L of DFMO were administrated compared with that of control group, and this variation was obvious with DFMO dosage increasing. The same was observed with eosin dying cell counting. Tunel method showed that apoptosis was induced by DFMO with 4 mmol/L, 6 mmol/L, and 8 mmol/L, but 6 mmol/L seems to be a suitable dosage. All these methods showed that if exogenous putrescence was added simultaneously, there was no difference significantly compared with that of control group both in cell proliferation and apoptosis. Conclusion The proliferation of bladder carcinoma EJ cell could be inhibited by DFMO and also apoptosis could be induced by DFMO.