Expression of HSP70 mRNA Induced by Chlorpyrifos in Rat Hepatic Tissue / 环境与健康杂志

ABSTRACT

Objective To understand the expression of heat shock protein(HSP70)mRNA induced by chlorpyrifos in the hepatic tissue of rats.Methods Wistar rats aged 6 weeks were divided into 4 groups,12 in each including 6 male and 6 female,the rats in the experimental groups were treated with chlorpyrifos by gavage once a day at the doses of 17 mg/kg?bw,8.5 mg/kg?bw and 4.25 mg/kg?bw respectively for 14 consecutive days,one group was the negative control.After the exposure,the hepatic tissue was sampled to examine the expression of HSP70 mRNA by polymerase chain reaction(PCR).Results The expression of HSP70 mRNA in the hepatic tissue in the groups of 17 mg/kg?bw,8.5 mg/kg.bw was significantly up-regulated with a dose-response relationship compared with the negative control group.Conclusion The expression of HSP70 mRNA is a sensitive parameter for early exposure assessment of chlorpyrifos.