Study on the NF-?B activation and mRNA expression of NF-?B,IL-1?,TNF-? and IL-6 in PBMCs of first-episode schizophrenia / 中国免疫学杂志

ABSTRACT

Objective:To measure the nuclear factor kappa B(NF-?B) activation and its mRNA expression in the PBMC,and to analyze the interaction between NF-?B activation and IL-1?,IL-6,TNF-? mRNA expression for exploration the role of NF-?B in production of cytokine in schizophrenia.Methods:Transcription Factor Assay Kits were used to measure NF-?B activation.RT-PCR technique was perftormed to analyze semiquantitatively NF-?B,IL-1?,IL-6 and TNF-? mRNA expression in the PBMC in both schizophrenia and control group.Results:NF-?B activation and its mRNA expression in the schizophrenia group were significantly higher than in the control group(P0.05).IL-1?,IL-6 and TNF-? mRNA expressions in the PBMCs from the schizophrenia group were significantly higher than those from the control subjects(P0.05).Conclusion:It is of significance to measure NF-?B activation in evaluating the regulation function of NF-?B.Activated NF-?B plays an important role in mediating the expression of IL-1? and TNF-? gene in schizophrenia.