A study of corneal preservation with closed Dexsol medium / 眼科

ABSTRACT

An experimental study was made on the preser- vation of donor corneas with a Dexsol medium prepared,a total of 40 animal corneas(rabbit and monkey,20 each)and 5 human corneas were used.The corneas were preserved separately at 4℃ for 2 weeks and at 34℃ for 5 weeks.The corneas preserved at different temperatures for different lengths of time were doubly stained with trypan-blue and alizarin red,which revealed that the endothelial cells remained fully active.Transmisscon and scanning election microscopy showed that neither the corneas preserved at 4℃ for 2 weeks nor those preserved at 34℃ for 5 weeks had any pathological changes in the ultrastructure of their endothelial cells.The rabbit cornea preserved with the Dexsol medium was used in homoplastic perforating keratoplasty,which resulted in a transparent transplant 2 months after the opera- tion.In 20 patients who received donor corneas preserved in the Dexsol medium for keratoplasty, the grafts remained clear 3 months after operation, and had an average endothelial cellular density of 1915/m~2 in the central region.