SOLUBLE EXPRESSION OF ? AND ? GENES OF HUMAN HEMOGLOBIN IN ESCHERICHIA COLI / 解放军医学杂志

ABSTRACT

Fragments of ? and ? genes were cloned into expression plasmid pET-21b. Bacteria harbouring expression plasmid was induced with IPTG,and a band of expressed protein of 16 kD was found in PAGE. They expressed as soluble products. ? expression product reached about 5% of total bacterial protein,? expression product reached about 15% of total bacterial protein. The products were confirmed by Western-blotting.