PRELIMINARY STUDY OF THE MOLECULAR STRUCTURE OF GLOB-N:A GLIOMA INHIBITOR / 解放军医学杂志

ABSTRACT

In order to study the molecular structure of Glob-N, a glioma inhibitor, Glob-N was separated from the membrane of human type O red blood cells, cow brain and normal human brain tissue respectively. Their structures were identified by using time-of-flight mass spectrometry, 1H-NMR and 13C-NMR. The results showed that the structure of Glob-N from the membrane of human type O red blood cells was proposed as globotetraosylceramide, while Glob-N from cow brain and normal human brain was globotriaoceramide, and the number of carbon atoms in the ceramide of the Glob-N was 27, 33 and 26 respectively. It suggested that Glob-N is a kind of neutral glycosphingolipids, and its molecular structure is different in different tissues.