Ocular Toxicity of Miconazole in Vitreous Replacement Fluid

ABSTRACT

To determine the ocular toxicity of intravitreal miconazole, we conducted a controlled trial in 20 pigmented rabbit eyes(10 rabbits). An anterior chamber paracentesis was performed on all animals and 0.4 ml of perfluoropropane(C3F8) gas was injected intravitreally. On day three, when it was fully expanded, eyes were underwent C3F8/fluid exchange with vitreous replacement fluid. The infusion solution contained various amounts of miconazole, 0.5 microgram/ml. 1 microgram/ml, 5 microgram/ml, 10 microgram/ml, 50 microgram/ml, 100 microgram/ml and normal saline for control. Intravitreal infusion was performed for 25-30 minuteds and a total of 100-120 ml of fluid was replaced. Histopathological examination revealed retinal toxic reactions in concentrations of 10 microgram/ml or greater. This study suggests that infusion solution of 5 microgram/ml or less is the concentration exceeds the minimal inhibitory concentration for most of fungi and may be safetly used in the treatment of fungal endaphthalmitis.