PREPARATION AND CHARACTERIZATION OF MONOCLONAL ANTIBODY AGAINST SYNTHETIC PEPTIDES OF HUMAN TOLL-LIKE RECEPTOR 4 / 解放军医学杂志
Article
de Zh
| WPRIM
| ID: wpr-552483
Bibliothèque responsable:
WPRO
ABSTRACT
An immunogenic peptide sequence of B cell dominant epitope of human Toll like receptor 4 (TLR4) was selected basing on predication of B cell epitope of TLR4.bALB/c mice were immunized with synthetic peptide of TLR 4 and one clone (B4) of hybridoma cells was picked out by ELISA and limited dilution. High titer McAb prepasations against TLR 4 were obtained from ascites fluid of BALB/c mice infravenously inoculated with the McAb inducing hybridoma cells.The titer of ascites fluid was 1∶ 100 000 .The subclass of the McAb was IgG2,k.The specificity of McAb was identified. by indirect ELISA,and a single strong blot was shown in molecular weights of 90 Kd.using Western blot analysis.
Texte intégral:
1
Indice:
WPRIM
langue:
Zh
Texte intégral:
Medical Journal of Chinese People's Liberation Army
Année:
1982
Type:
Article