EFFECTS OF PROTEIN FROM TRICHOLOMA MATSUTAKE MYCELIUM ON APOPTOSIS OF HeLa CELLS / 营养学报

ABSTRACT

Objective: To study the antitumor effect of the protein from Tricholoma matsutake against HeLa cells in vitro. Methods: Active protein was separated from the mycelium after sub-merged fermentation, and the antitumor effect against HeLa cells was studied in vitro by cell growth inhibition, scanning electron microscope, flow cytometry and DNA electrophoresis. Results: The mycelium protein-TMP-B had strong antitumor effect and induced apoptosis in vitro. TMP-B inhibited cell proliferation and induced cell apoptosis by arresting cell conversion from S to G 2/M state. HeLa cells exposed to TMP-B showed apoptotic peaks. Agarose gel electrophoresis of DNA from cells treated with TMP-B revealed "DNA ladder". Conclusion: The mycelium protein from Tricholoma matsutake can inhibit cervix cancer cells--HeLa cell proliferation and induce apoptosis.