Etiological role of polymorphism IVS+9C→G in hMSH2 gene in gastric cancers / 医学研究生学报

ABSTRACT

Objective: To investigate the etiological role of polymorphism IVS1+9C→G in hMSH2 gene in gastric cancers. Methods: A case-control study has been taken on subjects included 72 sporadic gastric, 71 familial gastric cancers and 126 healthy individual controls. Genomic DNA was extracted from peripheral white cell of all subjects. The polymorphism was detected by a PCR-based DHPLC analysis and verified by DNA sequencing. Results: The polymorphism IVS1+9C→G in hMSH2 gene was detected in 33.3%(42/126) Healthy individuals, 40.3%((29/72 ))sporadic gastric and 43.7%(31/71 )familial gastric cancers. Significant difference existed between cancers at young age (