Screening of binding protein of hepatitis C virus NS4A by phage display from human liver cDNA library / 解放军医学杂志
Medical Journal of Chinese People's Liberation Army
;
(12)1982.
Article
Dans Chinois
| WPRIM
| ID: wpr-554634
ABSTRACT
Objective To screen the HCV NS4A binding protein. Methods By using HCV NS4A as a solidified selective molecule, the T7 select human liver cDNA library was biopanned and the positive clones were selected. After screening, the positive plaques was amplified and then cloned into the pGEM-Teasy vector. Two positive plaques were chosen for DNA sequencing. Results The binding protein of HCV NS4A was identified as mitogen-activated protein kinase (MAPK)-activated protein kinase 5 (MAPKAPK5) by BLAST. Conclusion This approach provides a new way for the study of the pathogenic mechanism of HCV infection.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Type d'étude:
Etude diagnostique
/
Étude de dépistage
langue:
Chinois
Texte intégral:
Medical Journal of Chinese People's Liberation Army
Année:
1982
Type:
Article
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