Reversal of multidrug resistance by CPUE1 in K562/A02 cells / 中国临床药理学与治疗学
Chinese Journal of Clinical Pharmacology and Therapeutics
;
(12)2002.
Article
Dans Chinois
| WPRIM
| ID: wpr-554981
ABSTRACT
AIM:
To study the effect of CPUE1 on the reversal of multidrug resistance (MDR) in K562/A02 cells and its mechanism.METHODS:
MTT assay was used to determine the influence of CPUE1 on the cytotoxicity of vincrisine (VCR) in K562/A02 cells. The effect of CPUE1 on the apoptosis induced by VCR in K562/A02 cells was detected using DNA analysis and Annexin-Ⅴ/PI double stain assay. Flow cytometry was used to investigate the efflux of rhodamine123 (Rh123) by P-glycoprotein (P-gp) in K562/A02 cells.RESULTS:
CPUE1 increased the cytotoxicity and apoptosis induced by VCR in K562/A02 cells. At the concentration of 10 ?mol?L -1 ,CPUE1 reduced the IC_ 50 value of VCR from 60.54 to 4.17 ?mol?L -1 . CPUE1 inhibited the efflux of Rh123 and resulted in the increase of intracellular accumulation of Rh123 in K562/A02 cells.CONCLUSION:
CPUE1 has strong reversal effect on MDR in K562/A02 cells by inhibiting P-gp activity.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Chinese Journal of Clinical Pharmacology and Therapeutics
Année:
2002
Type:
Article
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