Experimental investigation on influence of proliferation of HSC by the serum containing Fufangbiejiaruanganfang herbs / 解放军医学杂志

ABSTRACT

Objective To study the effects of TGF-? 1 on the proliferation of hepatic stellate cell(HSC) and the interfering effect of serum containing Fufangbiejiaruanganfang (FFBJRGF ) drug in vitro. Methods TGF-? 1 was added to the culture of HSC in vitro and the proliferation of HSC was detected by MTT method. The effects of the serum containing different dosage FFBJRGF on the HSC proliferation was observed by using modified method of serum pharmacology of the traditional Chinese medicine and MTT method. Results The proliferation of HSC was decreased under adding exogenous TGF-? 1, which effect was presented at 72 hours after TGF-? 1 complement. The HSC proliferative reactions were obviously inhibited by adding sera containing high, moderate, low dosage of FFBJRGF as well as IFN-?serum compared with the normal control HSC culture (P