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Glucosides of Chaenomeles speciosa suppressed contact hypersensitivity response via modulating the thymus T lymphocytes subsets in mice / 中国药理学通报
Chinese Pharmacological Bulletin ; (12)2003.
Article Dans Chinois | WPRIM | ID: wpr-555473
ABSTRACT
Aim To investigate the effects and mechanisms of glucosides of chaenomeles speciosa (GCS) in mice with contact hypersensitivity (CHS) response. Methods CHS model in mice induced by 2,4-dinitro-I-dinitroflurobenzene (DNFB) was used in this study. Concanavalin A (Con A)-induced splenocytes proliferation was measured by the MTT colorimetric method and interleukin-2 (IL-2) activity was measured by testing its ability to support ConA-induced mice splenocytes proliferation by MTT method. Interleukin-4 (IL-4) and transforming growth factor -beta 1 (TGF-? 1) levels were determined by ELISA. T lymphocytes subsets were measured by flow cytometry. Results Similar as the control drug 4-acetylaminophenylacetic acid (actarit or Acta) (120 mg?kg -1), GCS (240 mg?kg -1) could inhibit the thymus index and spleen index in CHS mice. GCS( 60,120,240 mg?kg -1) could inhibit the ear swelling of CHS mice and could inhibit the splenocytes proliferation induced by ConA. GCS (240 mg?kg -1)decreased CD4 +CD8 +T lymphocytes subsets ratio and resumed the CD4 +CD8 -subsets ratio in CHS mice;GCS(240,60 mg?kg -1) resumed the CD4 -CD8 -subsets ratio in CHS mice. GCS also decreased the TGF-? 1 and IL-2 levels and increased the IL-4 levels in mice thymus with CHS. Conclusion GCS could inhibit mice CHS reaction and resume the balance of T lymphocytes subsets in mice thymus with CHS, and also could modulate the cytokines production by CD4 + T lymphocytes of CHS mice.

Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Type d'étude: Étude pronostique langue: Chinois Texte intégral: Chinese Pharmacological Bulletin Année: 2003 Type: Article

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Texte intégral: Disponible Indice: WPRIM (Pacifique occidental) Type d'étude: Étude pronostique langue: Chinois Texte intégral: Chinese Pharmacological Bulletin Année: 2003 Type: Article