The influence of mesenchymal stem cell on T lymphocyte transformation / 解放军医学杂志

ABSTRACT

Objective To study the immunoregulatory ability of mesenchymal stem cell (MSC) on T cells. Methods MSC were isolated and cultured from bone marrow and CD3 + T cells from peripheral blood. Then the action of MSC on T cell proliferation was investigated. ELISA assay tested IFN-? and IL-10 secretion of CD3 + T cells. Results The data showed that MSC inhibited T cell proliferation in a dose dependent manner when there was 2?10 5/well (96-well plate) CD3 +T cells. While MSC stimulated T cell proliferation when there was 5?10 4/well CD3 + T cells. High dose CD3 +T cells produced high level IFN-? and IL-10 in the absence of MSC, while when there were MSC in the culture, 5?10 4/well CD3 +T cells secreted more IFN-? and IL-10 than 2?10 5/well CD3 + T cells. Conclusion The result suggested that the effect of MSC on T cells was complex, not only correlating with the cell concentration of MSC but also of T cells.