EFFECT OF GENISTEIN ON BONE METABOLISM IN OVARIECTOMIZED RATS / 营养学报

ABSTRACT

Objective: To investigate the effects of genistein (Gen) on bone turnover in ovariectomized rats. Methods: Forty-seven Wistar rats were randomly allocated into six groups sham-operated (sham), ovarietomized (ovx), ovarietomized plus diethyl stilbestrol [E, 20?g/(kg bw?d)] or Gen [25、50、100 mg/(kg bw?d)] administration. After the rats had been fed for 3 mo,bone mineral density (BMD), histomorphometric parameters, the serum concentration of osteocalcin (BGP) and urinary contents of pyridine and hydroxyproline (HYPRO) were analysed. Results: BMD, trabecular bone volume (TBV) and mean trabecular plate density (MTPD) of ovariectomized rats were significantly decreased, but serum concentrations of BGP,urinary contents of deoxypyridinoline and HYPRO and mean trabecular plate space (MTPS) increased compared with the sham. After 3 mo of Gen supplementation, TBV,MTPD and urinary deoxypyridinoline were improved significantly in ovariectomized rats. Conclusion: Genistein reduced bone loss in ovariecromized rats by suppressing bone metabolism and osteoclast activity.