Partial mediation of GABA_A receptor in the antinociceptive stimulation of propofol at spinal level in rats / 中国药理学通报

ABSTRACT

Aim To observe the effects of propofol at spinal level on nociceptive response in rats and the possible role of GABA_A receptor.Methods In the praxiology test,Sprague-Dawley(SD) female rats were randomized into groups.Bicuculline and propofol were microinjected into intrathecally(ith).The noxious response was evaluated with hot plate and formalin test.In immunohistochemistry test,Fos-like immunoreactivity(FLI) neurons expressed in spinal dorsal horn(DH) induced by formalin intraplantar injection(sc) of one hindpaw were used as a neuroactive marker to observe the effects of propofol on the noxious transmission in DH.Results In hot-plate test,significant analgesia produced by propofol(10 g?L~(-1)) was antagonized about 81%,55%,81% and 97% at 10,20,30 and 40 min by ith bicuculline(0.01 g?L~(-1),P