Changes of Akt mRNA expression in rat pulmonary arterial smooth muscle cells induced by hypoxia / 第三军医大学学报

ABSTRACT

Objective To observe the changes of Akt1,Akt2,Akt3 mRNA expression level in rat pulmonary arterial smooth muscle cells(PASMC) induced by hypoxia and to investigate the value of Akt signaling pathway in vascular reconstruction during phyoxia pulmonary hypertension(HPH).Methods The pure PASMCs were established by tissue-sticking methods.Semi-quantitative reverse transcription polymerase chain reaction(RT-PCR) were used to examine the Akt1,Akt2 and Akt3 mRNA expression after PASMCs were exposed to normoxia(21%O_2) or hypoxia(3%O_2) for 2,8,12 and 24 h respectively.Results The pure PASMCs were established.The mRNA of Akt1,Akt2,Akt3 could be detected in all groups.Exposed to hypoxia,the mRNA expression levels gradually increased,reached the peak at 8 h,then decreased,and at 24 h down to the level under normoxia.As compared with that exposed to normoxia,the mRNA expression levels of Akt1 at 2,8,12,24 h,Akt2 at 8 h,Akt3 at 8 h in PASMCs exposed to hypoxia showed significant difference(P