Differential Expression of Peroxiredoxin Isotypes in Cultured Cells from Human and Rat Skin / 대한피부과학회지

ABSTRACT

BACKGROUND: Peroxiredoxin(Prx) is a novel peroxidase family to remove hydrogen peroxide using thioredoxin system, which is consisted of thioredoxin, thioredoxin reductase and NADPH. Several enzymatic antioxidants, such as superoxide dismutase, catalase and peroxidases are known to be present in the normal skin. But very little is known on the expression of Prx in the normal skin. OBJECTIVE: The aim of this study was to evaluate the expression of Prx isotypes in the normal human and rat skin, and thus to understand the role of Prx in the skin. MATERIALS AND METHODS: In this study, expression of 5 isotypes of Prx was evaluated in the primary cultures of keratinocytes and fibroblasts from human and rat, HaCaT cells and A431 cells by Western blot analysis. Also, immunohistochemical study for Prx I-IV expression was performed in the rat fibroblasts. RESULTS: Western blot analysis provided strong signals for Prx I, II and III with the cell extracts of cultured cells from the human and rat. The signals for Prx IV were weakly positive in hK, A431, hF and rF. The signals for Prx VI were positive in human cells, but were negative in rat cells. The finding were also identified in the intact skin. From immunocytochemical study for Prx I-IV, they were stained positively as a reticulated pattern in the cytoplasm of rF without isotype-specific difference. The positive reaction was strong in perinuclear cytoplasm. CONCLUSIONS: This study shows that Prx is ubiquitously expressed in the normal human and rat skin with an isotype-specific expression by species and cell types.