The role of pulmonary surfactant A in corticosteroids regulating costimulatroy molecules on dendritic cells in murine asthma model / 解放军医学杂志

ABSTRACT

Objective To explore the mechanisms of corticosteroids regulating costimulatroy molecules on dendritic cells from mouse asthma model, and the role of SP-A in the process. Methods Murine asthma model was established with ovalbumin(OVA) sensitization and challenge, and the model was confirmed by histological analysis of lung tissues. Rabbit antibody against mouse, immunohistochemical ABC method and glucose-DAB-nickel technique for staining and computer image analysis is used to check the expression of pulmonary surfactant A (SP-A) in three groups. FACS was also used to measure the expression of CD-80 on spleen derived dendritic cell from OVA-sensitized and challenged mice. Data was analysised with spss 10.0 software. Results Histological analysis of lung tissues was consistent with the characteristic of murine asthma model. The expression of SP-A in small trachea of asthma group was decreased vs. control group and dexamethasone group(P