Up-regulation of osteopontin gene in BALB/c 3T3 cells induced by tumor promoters of TPA,okadaic acid and cadmium chloride / 第三军医大学学报
Journal of Third Military Medical University
;
(24)2003.
Article
Dans Chinois
| WPRIM
| ID: wpr-558881
ABSTRACT
Objective To explore the changes of osteopontin (OPN) gene expression induced by tumor promoter of 12-O-tetradecanoylphorbol13-acetate(TPA),okadaic acid(OA) or cadmium chloride(CdCl_(2)).Methods The two-stage transformation test of BALB/c 3T3 cells was established with MNNG as initiator,TPA,OA or CdCl_(2) as promoter respectively.Mouse toxicology gene chip was used to detect the gene expression of BALB/c 3T3 cells transformed with TPA,OA or CdCl_(2) respectively,and the expression of OPN gene was validated by real-time RT-PCR assay.Results TPA,OA or CdCl_(2) could increase the transcriptional expression of OPN gene in BALB/c 3T3 cells.The expression of OPN gene was up-regulated in the eight transformed cell colonies induced by TPA,OA or CdCl_(2) respectively.Moreover,the up-regulation of OPN gene expression was confirmed by real-time RTPCR assay.Conclusion The up-regulation of OPN gene expression is closely related to cell transformation of BALB/c 3T3 cells induced by TPA,OA or CdCl_(2).
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Journal of Third Military Medical University
Année:
2003
Type:
Article
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