The effect of overexpression of sphingosine kinase on biological characters of gastric carcinoma cells / 解放军医学杂志

ABSTRACT

Objective To investigate the roles of sphingosine kinase (SPK) on the regulation of proliferation, migration and apoptosis of gastric carcinoma cells. Methods BGC-823 cells were infected with adenoviral carriers bringing the wild type SPK gene and SPK mutant (SPK DN ), and their effect on the migration and 5-FU-induced apoptosis of BGC-823 cells was evaluated. The adenovirus-mediated expression of SPK was detected by Western blot, and cellular SPK enzyme activity was assayed. The cell migration was investigated by Transwell Migration Technology. Results The adenovirus-mediated wild type SPK gene increased the cellular SPK activity, thus leading to enhanced migration and resistance to 5-FU-induced apoptosis of BGC-823 cells. Whereas overexpression of SPK mutant (SPK DN ) decreased the cellular SPK activity, thus inhibited the migration and sensitized BGC-823 to 5-FU-induced apoptosis. Conclusion SPK plays important roles in the regulation of migration and apoptosis of gastric carcinoma cells, therefore it may be of a new target for tumor treatment.