Preparation of lutein from the extraction of Marigold flowers by biotransformation / 中国药理学通报

ABSTRACT

Aim To study preparation of lutein from the extraction Marigold flowers by biotransformation.Methods Screen bacterium for lutein esters biotransforming by studying on the biotransformation of lutein esters by Penicillium on tabulate revulsive culture medium.Porule of and Aspergillus niger Penicillium could transform lutein esters into lutein,but biotransformation of Penicillium was better than Aspergillus niger.Porule of Pleurotus ostreatus could not grow on revulsive culture medium,but Pleurotus ostreatus could grow on the culture medium and transformed lutein esters into lutein.Research of shaking flash fermentation of Penicillium for trans-forming lutein esters.When pH is 6.0,quantity of incubation is 2 bacteria plugs and fermentation time is 12～24 hours,the transformation is completed and the content of lutein is 91.6%(HPLC)。Result Prolonging the transformation time will improve the percent of lutein but the content of lutein decreased.Conclusion Biological transrormation can be used from calendula officinacis extract preparation.