Effect of sendai virus replication sequence on protein changes in immature dendritic cells / 第三军医大学学报

ABSTRACT

Objective To investigate the protein change in immature dendritic cells(DC) exposed to sendai virus replication sequence.Methods The total cellular proteins expressed in DC infected with Sev/△F or SeV were collected and quantitated,then separated by two-dimensional gel electrophoresis and visualized by silver staining.Digital images were analyzed by PDQuest software.The differentially expressed protein spots were picked,digested in gel and subjected to peptide mass fingerprinting using MALDI-TOF MS.Results Protein spots in 2-DE gel for DC treated by Sev/△F were more than that by SeV,among which a lot of proteins increased.Conclusion It was relevant to the replication sequence that proteins decreased in dendritic cells infected with sendai virus vector.