Experimental studies on calcium ion concentration and mitochondrial membrane potential in cultured myocyte of rat subjected to hypovolemia shock / 解放军医学杂志
Medical Journal of Chinese People's Liberation Army
;
(12)1982.
Article
Dans Chinois
| WPRIM
| ID: wpr-561061
ABSTRACT
Objective To explore the changes in Ca2+ concentration and mitochondrial membrane potential in cultured myocytes in rat subjected to hypovolemia shock.Methods 84 Wistar rats were randomized into two groupshypertonic solution resuscitation(HES,n=42)and 0.9% sodium chloride resuscitation(NS,n=42).At different timepoint(pre-shock stage,shock,post-resuscitation 5min,15min,30min,60min and 90min),the cultured cardiac myocytes,some were stained with JC-1,used fluo-4/AM as calcium probe,the others were assayed for Ca2+ mitochondrial membrane potential by flow cytomety.Results Ca2+ concentration of group HES at shock,post-resuscitation 5min,15min and 30min was higher than that of pre-shock stage,but mitochondrial membrane potential at post-resuscitation 60min and 90min was lower(P0.05).Ca2+ concentration and mitochondrial membrane potential of group HES at post-resuscitation 60min and 90min were more significantly different than that of group NS(P
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Type d'étude:
Essai clinique contrôlé
langue:
Chinois
Texte intégral:
Medical Journal of Chinese People's Liberation Army
Année:
1982
Type:
Article
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