Construction and identification of plasmid vector of short interfering RNA on pneumocystis carinii major surface gene / 重庆医科大学学报
Journal of Chongqing Medical University
;
(12)2003.
Article
Dans Chinois
| WPRIM
| ID: wpr-561113
ABSTRACT
Objective:
To construct and identify a plasmid vector of short interfering RNA(siRNA) on pneumocystis carinii(PC) major surface glycoprotein(MSG) gene.Methods:
Short hairpin RNA(shRNA) oligonucleotide targeting PC MSG gene which was chemically synthesized and inserted into RNAi-Ready plasmid vector pTZU6+1 after annealing.the recombinant plasmid, pPC-MSG,transgormed into E.coil.TOP10 and amplified,was digested by restriction endonucleases HindⅢand EcoRⅠand identified by gelelectrophoresis and DNA sequencing.Results:
Gel electrophoresis and DNA sequencing showed that the recombinant plasmid containing the correct and full shRNA oligonucleotide.Conclusion:
The siRNA plasmid,pPC-MSG was constructed successfully.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Type d'étude:
Etude diagnostique
langue:
Chinois
Texte intégral:
Journal of Chongqing Medical University
Année:
2003
Type:
Article
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