Evaluation of free fatty acid-induced insulin resistance with euglycemic-hyperinsulinemic clamping / 中国实用内科杂志

ABSTRACT

Objective To prove insulin resistance-induced by free fatty acid(FFA)with hyperinsulinemic-euglycemic clamp and assess the prevention from this resistance by N-acetylcysteine(NAC).Methods Twenty-four female Wistar rats were randomly divided into 4 groups,each of which received infusion of IH(20% intralipid +20 U/mL heparin),IH+NAC,NAC,and saline respectively for 7 h.Two-hour hyperinsulinemic-euglycemic clamp was made after 5h infusion.During the infusion and clamp the rats were awake.Blood samples were taken for measuring insulin,FFA and maleic dialdehyde(MDA)at 0 min,280 min,290 min,300 min,340 min,380 min,400 min,410 min and 420 min.The insulin sensitive index was calculated.Results Plasma FFA and MDA levels in IH group were significantly elevated compared with those in control,IH+NAC and NAC groups,P