A method for detection of HER2 gene amplification in breast cancer tissues:chromogenic in situ hybridization method and its modification / 第二军医大学学报
Article
Dans Zh
| WPRIM
| ID: wpr-562878
Responsable en Bibliothèque :
WPRO
ABSTRACT
Objective:To apply chromogenic in situ hybridization(CISH)for detection of HER2 gene amplification in breast cancer tissues and to discuss some modifications of the CISH method.Methods:HER2 gene amplification was detected by CISH in 60 breast cancer specimens with an immunohistochemical score over 2+.The correlation between the results of IHC and CISH was analyzed.Our experience in CISH manipulation was summarized and optimization to CISH was discussed.Results:CISH identified gene amplification in 91%(40/44)specimens with an IHC score of 3+ and in 50%(8/16)specimens with an IHC score of 2+.The total concordance rate between IHC and CISH was 80%(48/60,P
Texte intégral:
1
Indice:
WPRIM
Type d'étude:
Diagnostic_studies
/
Prognostic_studies
langue:
Zh
Texte intégral:
Academic Journal of Second Military Medical University
Année:
1982
Type:
Article