esp1 expression and its effect on ploidy in A549 and Hela cells / 第三军医大学学报
Journal of Third Military Medical University
;
(24)2003.
Article
Dans Chinois
| WPRIM
| ID: wpr-563320
ABSTRACT
Objective To observe the relation of estradiol-stimulated protein (esp1) expression with chromosome ploidy in A549 and Hela cells. Methods Total RNA was extracted from A549 and Hela cells respectively for cDNA. pIRE-S2-EGFP-ESP1 plasmid was transfected into the 2 corresponding tumor cells. Real-time quantitative PCR was used to detect esp1 gene expression. The chromosome changes were analyzed by VIDEOTEST-KARYO 3.1 and chromosome ploidy was analyzed with flow cytometry. Results After the tumor cells were transfected with pIRE-S2-EGFP-ESP1 plasmid, the expression of esp1 gene was up-regulated (P
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Journal of Third Military Medical University
Année:
2003
Type:
Article
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