Growth inhibition effects of jasmonates on human neuroblastoma cell line SH-SY5Y and its mechanisms / 中国药理学通报
Chinese Pharmacological Bulletin
; (12)1986.
Article
Dans Zh
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| ID: wpr-563378
Responsable en Bibliothèque :
WPRO
ABSTRACT
Aim To explore the growth inhibition effects of jasmonates on human neuroblastoma SH-SY5Y cell line,and to investigate its mechanisms.Methods After administration of 0.5~2.5 mmol?L-1 jasmonates for 6~24 hrs, the growth inhibition rates of SH-SY5Y cells were studied by MTT colorimetry.Cell cycle phases were assayed by propidium iodide staining flow cytometry. Cellular apoptosis was inspected by Hoechst 33258 fluorescent staining and Annexin V-FITC and propidium iodide staining flow cytometry.Gene expressions of PCNA, cyclin D1 and N-myc were determined by reverse transcription polymerase chain reaction.Results Jasmonates inhibited the growth of SH-SY5Y cells in a dose-and time-dependent manner,while the methyl jasmonate was the most efficient. After administration of 0.5 to 2.5 mmol?L-1 of methyl jasmonate for 24 hrs,the growth inhibition rates of cells reached 5.75%~88.7%(P
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Indice:
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langue:
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Texte intégral:
Chinese Pharmacological Bulletin
Année:
1986
Type:
Article