Changes in the expressions of CD63 on basophils in patients with penicillin allergy / 中国药理学通报

ABSTRACT

Aim To evaluate the value of expressions of CD63 on basophils in diagnosing allergy to penicillins.Methods The expressions of CD63 on basophils activated with 9 kinds of antigens(PGpenicillin G,PVphenoxomethylpenicillin,AMPampicillin,AXamoxicilloyl,6-APA6-aminopenicillanic acid,PHAphenylacetic acid,PHOAphenoxacetic acid,PHPGN-(?-hydroxyphenyl)glycine,NPGN-phenylglycine) were detected by FAST(Flow cytometric allergen stimulation test) in 43 patients with penicillins allergy.Eight types of specific IgE and IgG antibodies to penicillins were determinated by RAST and ELISA,respectively.Results The sensitivity and specificity of CD63 used as a marker in patients with penicillins allergy were 65.12% and 93.33%,respectively.Moreover,the sensitivities of CD63 and specific IgE were higher than those of specific IgG in patients with positive skin test(P