Development of a multiplex PCR assay to detect Edwardsiella tarda, Streptococcus parauberis, and Streptococcus iniae in olive flounder (Paralichthys olivaceus)
Journal of Veterinary Science
;
: 163-166, 2014.
Article
Dans Anglais
| WPRIM
| ID: wpr-56420
ABSTRACT
A multiplex PCR protocol was established to simultaneously detect major bacterial pathogens in olive flounder (Paralichthys olivaceus) including Edwardsiella (E.) tarda, Streptococcus (S.) parauberis, and S. iniae. The PCR assay was able to detect 0.01 ng of E. tarda, 0.1 ng of S. parauberis, and 1 ng of S. iniae genomic DNA. Furthermore, this technique was found to have high specificity when tested with related bacterial species. This method represents a cheaper, faster, and reliable alternative for identifying major bacterial pathogens in olive flounder, the most important farmed fish in Korea.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Sujet Principal:
Infections à streptocoques
/
Streptococcus
/
Poissons plats
/
Sensibilité et spécificité
/
Edwardsiella tarda
/
Infections à Enterobacteriaceae
/
Réaction de polymérisation en chaine multiplex
/
Maladies des poissons
/
Pêcheries
Type d'étude:
Etude diagnostique
/
Étude pronostique
Limites du sujet:
Animaux
langue:
Anglais
Texte intégral:
Journal of Veterinary Science
Année:
2014
Type:
Article
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