Effects of the crude extracts of Abrotani herba on the activity of NF-?B in colon carcinoma HT-29 and Lovo cells / 解放军医学杂志

ABSTRACT

Objective To study the NF-?B activity in colon carcinoma HT-29 and Lovo cells treated with different crude extracts of Abrotani herba obtained by column chromatography with macroporous resin.Methods The decoction of Abrotani herba absorbed by macroporous resin AB-8 was mounted into the column and then eluted with distilled water and 30%,50%,75% and 95% alcohol.To select appropriate concentrations for treatment,HT-29 cells were pretreated for 24 hours with each elution phase of the rude extracts in different concentrations(0,50,100,200,400 and 600?g/ml),and then their activity was detected by MTT.The HT-29 and Lovo cells were thereafter cultured in DMEM complete media respectively containing distilled water extract and 30%,50%,75% and 95% alcohol extracts in the concentrations as obtained by MTT assay,and the cells in control group were cultured in DMEM only.The cells were then harvested and the nucleic proteins were extracted for measurement with electrophoretic mobility shift assay(EMSA).Changes in NF-?B activity in HT-29 and Lovo cells treated with different concentrations of crude extracts were observed by EMSA.Results Viability of HT-29 cells treated with 400 and 600?g/ml crude extracts were significantly lower than those treated with 0-200?g/ml crude extracts(P0.05).Conclusion Crude extracts of Abrotani herba,extracted by column chromatography with macroporous resin and eluted with 30% alcohol,can inhibit NF-?B activity in colon carcinoma HT-29 and Lovo cells.