Determination of Magnob1 and Honokiol in Baolong Pill By HPLC / 浙江中医药大学学报

ABSTRACT

[Objective]To establish an HPLC method for the determination of magnolol and honokiol in Baolong Pill.[Methods]The samples were analyzed on an Agilent C18 column(4.6mm?250mm,5?m).The mobile phase was a mixture of MeOH-H2O (78∶22);the rate of flow was 1.0 m1/min;the detection wave-length was 294 nm.[Results]Calibration graph was linear in the range of 0.02~0.26?g,r= 0.999 9 for magnolol;0.012~0.156?g,r= 1 for honokiol.The recovery was 100.46%,RSD=1.00% for magnolol;100.76%,RSD=1.37% for honokiol.[Conclusion] The method is sensitive and its results are accurate with good reproducibility.It can be used for the quality control of Baolong Wan.