Molecular cloning, purification and immunogenicity of recombinant Brucella abortus 544 malate dehydrogenase protein
Journal of Veterinary Science
; : 119-122, 2016.
Article
Dans En
| WPRIM
| ID: wpr-56503
Responsable en Bibliothèque :
WPRO
ABSTRACT
The Brucella mdh gene was successfully cloned and expressed in E. coli. The purified recombinant malate dehydrogenase protein (rMDH) was reactive to Brucella-positive bovine serum in the early stage, but not reactive in the middle or late stage, and was reactive to Brucella-positive mouse serum in the late stage, but not in the early or middle stage of infection. In addition, rMDH did not react with Brucella-negative bovine or mouse sera. These results suggest that rMDH has the potential for use as a specific antigen in serological diagnosis for early detection of bovine brucellosis.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Protéines recombinantes
/
Brucella abortus
/
Brucellose
/
Test ELISA
/
Maladies des bovins
/
Clonage moléculaire
/
Escherichia coli
/
Malate dehydrogenase
/
Antigènes bactériens
Type d'étude:
Screening_studies
Limites du sujet:
Animals
langue:
En
Texte intégral:
Journal of Veterinary Science
Année:
2016
Type:
Article