Effects of Different Processing on the Contents of Paeono1 and Paeoniflolin in Cortex Moutan / 医学研究杂志

ABSTRACT

Objective A HPLC method was established for the determination of paeonol and paeoniflolin in cortex moutan from different processing.To investigate the necessity of processing to the cortex moutan.Methods The HPLC(high performance liquid chromatogram)method was established for the determination of the contents of the paeonol and paeoniflolin in cortex moutan.The conditions of the chromatograph method was as followingAC18 column was used.The mobile phase for paeonol determination was methanol-water(4555)and detection wavelength was 274nm.The mobile phase for paeoniflorin determ ination was acetonitrile-0.1%H3PO4(1585)and detection wavelength was 230nm.Results The results showd that the linear ranges of paeonol and paeoniflorin determ ination were 0.36～2.2?g/L～and 0.042～1.06?g/L respectively.The average recoveries of paeonol and paeoniflorin were 96.30%(RSD=0.97%)and 98.21%(RSD=0.64%)respectively.Conclusion The method is simple,accurate and reproducibility.The experimental results showd that the contents of paeonol and paeoniflorin in coaex moutan from diferent processing were positive correlated,while the contents of cortex moutan from Sichuan were higher than that of the others.We had better combine the pharmacodynamic action study to illuminate the feasibility of processing to the Cortex Moutan.