Effect of cyclooxygenase-2 on injury to intestine induced by ischemia reperfusion in rat / 解放军医学杂志

ABSTRACT

Objective To investigate the effect of cyclooxygenase-2(cox-2)on prostaglandin I2(PGI2)/thromboxane A2(TXA2)system in ischemia reperfusion(I/R)injury to small bowel in rat.Methods Forty-eight male SD rats were randomly divided into three groupscontrol group(n=8),I/R group(n=20)and intervention group(n=20).Small intestine I/R model was established with the animals of I/R groupafter anesthesia,rats were split along the middle abdominal incision,the mesenteric artery was then bluntly separated and clipped with a blood vessel forceps at its root for 45 minutes to block the blood flow,then releasing the forceps for reperfusion.Animals of intervention group were lavaged with Nimeshulide 1h before operation,the remaining steps were the same as did in I/R group.The samples of blood and intestinal tissue were collected at 3,6,12 and 24 hours after operation.The content of plasma D-lactate was detected by ultraviolet spectrophotometric method.Radioimmunoassay was used to detect 6-keto-PGF1?(the stable metabolite of PGI2,reflected the level of PGI2)and thromboxane B2(TXB2,stable metabolite of TXA2,reflected the level of TXA2)in serum and tissues of small bowel.The expression of cox-2 mRNA was detected by RT-PCR.Results Compared with the control group,the levels of plasma D-lactate,cox-2,6-keto-PGF1? and TXB2 were significantly increased in I/R group,with more obvious increase of TXB2,and the value of 6-keto-PGF1?/TXB2 decreased significantly(P