Incubation of human ovarian cancer microvascular endothelial cells and formation of lumen-like structure in vitro / 第三军医大学学报
Journal of Third Military Medical University
;
(24)1983.
Article
Dans Chinois
| WPRIM
| ID: wpr-567074
ABSTRACT
Objective To establish the magnetic activated cell sorting system(MACS)for isolation and incubation of human ovarian carcinoma-derived microvascular endothelial cells(ODMECs) and to observe their ability to form tubule-like structure(TLS) in vitro.Methods MACS was used to purify ODMECs with antiCD31 antibody and ODMECs were identified according to their morphological,biochemical and phenotypic characteristics.The ability of ODMECs to intake acetylated-low-density lipoprotein(DIL-acLDL) and Ulex europeaus agglutinin-1(UEA-Ⅰ) was assayed to observe the formation of TLS in vitro.Results Flow cytometry showed that the purity of ODMECs was up to 99.6%.The ODMECs were characterized by cobble stones,contact inhibition,expressed CD31,CD105 and FⅧ-Rag,and exhibited the ability to bind to UEA-1,intake DIL-acLDL and form TLS during incubation.Conclusion The MACS we established can be used to isolate and incubate human ODMECs and study the formation characteristics of TLS.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
Type d'étude:
Étude pronostique
langue:
Chinois
Texte intégral:
Journal of Third Military Medical University
Année:
1983
Type:
Article
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