Effect of SDF-1/CXCR4 axis to homing of laryngeal carcinoma cell Hep-2 / 第三军医大学学报

ABSTRACT

Objective To investigate the in vitro expressions of chamotatic factor CXCL12 [also known as stromal cell-derived factor 1 (SDF-1)],the receptor of the factor,Cys-X-Cys receptor 4 (CXCR4) and vascular endothelial growth factor C (VEGF-C) in human laryngeal carcinoma cell line Hep-2 and their roles in the homing of laryngeal carcinoma. Methods The expressions of CXCR4 mRNA and protein in Hep-2 cells was detected by RT-PCR and Western blotting,the expression of VEGF-C also was evaluated before and after incubated with SDF-1 (1,10,100 ng/ml) or the antagonist of CXCR4 AMD3100. Methythiazolyltetrazolium (MTT) assay was used to analyze the effect of different concentrations of SDF-1 on the proliferation of Hep-2 cells. Transwell invasion chamber and matrigel were used to evaluate the effect of various concentrations of SDF-1 and AMD3100 on the migration and invasion of Hep-2 cells. Results 1)The CXCR4 and VEGF-C were both overexpressed at mRNA and protein level in Hep-2 cells,and the expression of VEGF-C in Hep-2 cells was up-regulated with a concentration-dependent model,which was inhibited by CXCR4 antagonist (P