HISTOLOGICAL AND ENZYME HISTOCHEMICAL STUDIES OF THE RAT THYROID GLAND DURING EXPERIMENTAL GASTRIC ULCER / 解剖学报

ABSTRACT

Eighty adult male Wistar rats were randomly divided into three groups 1. In the experimental peptic ulcer group, 35 animals were induced to develop peptic ulcer by injecting 0.05 ml/kg of glacial acetic acid (more than 99%) into the submucosa of the stomach after laparotomy under aseptic conditions. 2.In the saline control group, 35 animals were injected with 0.05 ml/kg of saline into the submucosa of the stomach after laparotomy. 3.In the normal control group, 10 normal animals without any treatment were raised under the same condition as group 1 and group 2. The thyroid glands of three groups were taken at definite time intervals (1-28 days) after the operation. The right thyroids were prepared for cryostat sections after hexane quenching (-60℃) and subjected to the histochemical studies of acid phosphatase (AcP), alkaline phosphatase(AIP), a-glycerophosphate dehydrogenase (a-GPD), succinate dehydrogenase (SDH), glucose-6-phosphate dehydrogenase (G6PD), and nonspecific esterase (NsE). The left thyroids were fixed in Carnoy's fluid, stained with HE and subjected to histological study. The reactions of enzymes of the follicular cells in the group 2 were weaker than those in the group 3 (normal control) during the period of 2-21 days after the operation. The follicular cells became flattened and follicular lumens increased in size. These changes recovered to normal on the 28th day after the operation. In the follicular cells of group 1. In which peptic ulcer developed after injection of glacial acetic acid, the reaction of A1P was weaker than those in the group 3, but stronger than those in the group 2. The reations of a-GPD, SDH, and G6PD were stronger than those in the group 2, and as well as those in group 3. The reaction of AcP was stronger than those in the group 2 and group 3 during the 6-21 days after the operation. The follicular cells in the group 1 became flattened and the follicular lumens increased in size only during the period of 4-10 days after the operation and recovered on the 14th day after the operation. These findings suggested that the thyroid follicular cells of rat involved in the metabolic activities of the repair of gastric ulcer.