THE GASTRIC INNERVATION OF RAT: A FLUORESCENT DOUBLE LABELLING TECHNIQUE / 解剖学报

ABSTRACT

The present investigation was performed in fifteen adult rats using four fluorescent tracers, including 4'-6-diamidino-2-phenylindol. 2 HC1 (DAPI), nuclear yellow (NY), propidium iodide (PI), and fast blue (FB), which were divided into four sets PI-DAPI, PI-NY, DAPI-NY, and FB-NY. In each set two fluorescent tracers were used separately to label gastric corpus and pylorus antrum. In the medulla oblongata, the labelled cells in the dorsal motor nucleus of vagus (DmnX) were observed in every animal, but those in the nucleus ambiguus (nA) were only seen. in 11 cases and in the nucleus tractus solitarius (nTs) in 5 cases. In addition, labelled cells were also found in laminae V of the posterior grey columns of the thoracic (T6-9) spinal cords, spinal ganglia and sympathetic trunks. The results of counting and analysing the labelled cells of DmnX of both sides of 75 sections in four cases are as follows 1233 cells on the left, including 1143 labelled cells of gastric corpus (92.7%), 63 labelled cells of pylorus antrum (5.1%), 27 double labelled cells (2.2%); 1007 cells on the right, including 930 labelled cells of gastric corpus (92.3%), 41 labelled cells of pylorus antrum (4.1%), and 36 double labelled cells (3.7%). The results indicate that PI is not fitted for 'long distance labelling in peripheral nervous system; the density of vagus innervation of gastric corpus is larger than that of pylorus antrum; a part of the nerve endings of vagus postganglionic neurons are distributed both in gastric corpus and in pylorus antrum; a part of postganglionic neurons of sympathetic fibres are located at the sympathetic trunk.