STUDIES ON THE EFFECTS OF GOSSYPOL AND GLYCOSIDES TRIPTERYGIUM WILFORDII(GTW) ON DNA BY IN SITU NICK TPANSLATION METHOD / 解剖学报

ABSTRACT

The present study reported the effect of two male antifertility agents gossypol acetic acid and GTW on DNA of C3H10T1/2 mouse fibroblasts. Our results showed that the cells treated with gossypol or GTW at high concentration (2-3 ?g/ml) for 4 hours, show silver grains in their nuclei as much as the positive control group, N-methyl N′-nitro-N-nitrosoguanidine (MNNG) a known carcinogen. However, if the agents were used at moderate concentrations (0.5-1?g/ml), the silver grains were much less, if the concentrations of gossypol or GTW were of 0.1-0.3 ?g/ml, the silver grains were as less as the control group. In a colony-forming test, we found that the cells lost their proliferate ability, since no colonies could be formed, if gossypol or GTW were of high concentration; while at moderate or low concentrations, the colony-forming rate was as high as 8.1-10.5％. Taking all of these results into consideration, we suggest that high concentrations of gossypol or GTW can damage cell DNA severely, moderate concentration of the agents break cell DNA to a certain extent, but the cells can repair, while low concentration of gossypol or GTW exert no obvious effect on cells. The significance of these observations was briefly discussed.