Genotype distributions of the 5 sites SNPs in the 3 subtypes of beta adrenoreceptor / 重庆医科大学学报
Journal of Chongqing Medical University
;
(12)2003.
Article
Dans Chinois
| WPRIM
| ID: wpr-571703
ABSTRACT
Objective:
To study the genotype-by site distributions of SNP(single nucleotide polymorphisms)at the 5 sites in the 3 subtypes of beta adrenoreceptor(Beta-AR or B-AR).Methods:
DNA was extracted from the peripheral venous blood of 338 subjects using Blood DNA Mini Kit.The 5 sites SNP in the 3 subtypes of Beta-AR were genotyped by PCR-RFLP(polymerase chain reaction-restriction fragment length polymorphism) and allele specified PCR techniques.Results:
The natural distribution characteristics for SNP at the 5 sites in the 3 subtypes of Beta-AR of 338 subjects were gotten.The predominant phenotypes are as followsBeta1-AR wild homozygote Ser/Ser49(70%),Beta1-AR wild homozygote Arg/Arg389(63%),Beta2-AR heterozygote Arg/Gly16(80%),Beta2-AR heterozygote Gln/Glu27(50%),Beta3-AR wild homozygote Trp/Trp64(65%).Conclusion:
It is heterogeneous in distribution in different styles for the genotype-by site distribution of SNP at the 5 sites in the 3 subtypes of Beta-AR,which is the basis for further investigating the relationship among the distribution,gene function and diseases.
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Journal of Chongqing Medical University
Année:
2003
Type:
Article
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