Change of puerarin on the dynamic equilibrium of ET-NO and ET-ANF of diabetic vascular complicatioin induced by streptozotocin / 中成药

ABSTRACT

AIM: To investigate the effect of puerarin on the alteration of ET and ANF in plasma, NO and NOS in serum of diabetic rats induced by streptozotocin(STZ). METHODS: Rat modeling was set up by means of intraperitoneal injection of ST2 with a dose of 60mg?kg -1. Four days later, blood glucose was measured by glucose oxygen kit. On the basis of present documents, the rats that blood glucose was over 16.7mM were accepted as diabetic ones, and then were divided randomly into five groups diabetic mellitus (DM), aminoguanidine (AG 0.1g?kg -1,ig.), puel (0.5g?kg -1,ig.),pue2(0.25g?kg -1,ig.), pue3(0.125g?kg -1,ig.) and a normal group additionally. In order to set up a chronic model, we observed 12 weeks according to our past experiments. The FBS was measured at the twelfth week using the test kit. NO and NOS in serum were determined by checking their OD values, ET and ANF in plasma were determined by radio-immunization, respectively. RESULTS: In diabetic rats, Plasma ET and ANF levels increased obviously, NO and NOS levels decreased greatly (P