Establishment of a hyperinsulinemic-euglycemic clamp technique in conscious rats / 重庆医科大学学报

ABSTRACT

Objective:To establish a hyperinsulinemic euglycemic clamp technique in conscious rat,and to explore the effect of anesthesia and acute infusion of lipid on insulin sensitivity of rats.Methods:Rats divided into two groups were catheterized in the right jugular vein and left carotid artery and underwent a hyperinsulinemic-euglycemic clamp with or without infusion of lipid (Intralipid) and in unconscious and conscious state.Results:Infusion of lipid resulted in a decrease of insulin sensitivity by 30%( P