RNA interference c-erbB-2 and c-raf-1 genes expression combined transfection in the Tca8113 cell lines / 重庆医科大学学报
Journal of Chongqing Medical University
;
(12)2003.
Article
Dans Chinois
| WPRIM
| ID: wpr-572742
ABSTRACT
Objective:
To investigate the proliferating inhibition and the mechanisms of dsRNA interference c-erbB-2 and c-raf-1 genes expression combined transfection in the human tongue carcinoma Tca8113 cell lines.Methods:
There were 3 groups in our study,control group,normal control group and RNAi experimental group.At different time after liposome-mediated transfection,the cell proliferation,apoptosis,mRNA level,protein expressing level and cell cycle were observed by MTT,RT-PCR and electronic microscope.Results:
According to the results of RNAi experimental group,the OD-value were 0.073( P
Texte intégral:
Disponible
Indice:
WPRIM (Pacifique occidental)
langue:
Chinois
Texte intégral:
Journal of Chongqing Medical University
Année:
2003
Type:
Article
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