Subsequent Embryo Transfers (SET) on Day 2 and 5: It's Safety and Effectiveness / 대한불임학회지
Korean Journal of Fertility and Sterility
; : 165-172, 2000.
Article
de Ko
| WPRIM
| ID: wpr-57319
Bibliothèque responsable:
WPRO
ABSTRACT
OBJECTIVE: In vitro fertilization (IVF) and a prolonging the time of culture may be helpful in establishing a viable pregnancy through a selection effect. Some embryos do not develop beyond the 4-cell stage and some may not develop to the blastocyst stage. We have evaluated the safety of SET and the outcomes of pregnancy. METHODS: Sperms were treated with Ham's F-10 supplemented with 10% human follicular fluid (hFF). Oocytes or fertilized oocytes were cultured in Dulbecco's Modified Eagle Medium (DMEM) with 10% or 20% hFF respectively. Up to five oocytes were inseminated with approximately 200,000 sperm cells/2 ml in each well. Fertilization was examined in the following morning and fertilized oocytes were co-cultured until embryo transfer. Vero cells for co-culture were prepared in Tissue Culture Medium - 199 (TCM-199) with 10% fetal bovine serum. At the two to four cell and blastocyst on day 2 and day 5, embryo and blastocyst grading were evaluated. Pregnancy rate was determined after transfer of human embryos at the two to four cell stage on day 2 (Group I) or subsequent transfer of embryos on day 2 and at the blastocyst stage on day 5 (Group II). For statistically significant when rho value was less than 0.05. RESULTS: No differences was found in the fertilization between Group I(81.0%, 98/121) and Group II(81.8%, 180/220). In case of cleavage rate, no difference was found in Group I(95.9%, 94/98) and Group II(66.7%, 12/18) than in Group (26.3%, 5/19). CONCLUSION: The results of this study showed that SET is safe and effective, and significantly increases the pregnancy rate.
Texte intégral:
1
Indice:
WPRIM
Sujet Principal:
Ovocytes
/
Spermatozoïdes
/
Blastocyste
/
Cellules Vero
/
Fécondation in vitro
/
Liquide folliculaire
/
Taux de grossesse
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Techniques de coculture
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Aigles
/
Transfert d'embryon
Limites du sujet:
Female
/
Humans
/
Pregnancy
langue:
Ko
Texte intégral:
Korean Journal of Fertility and Sterility
Année:
2000
Type:
Article